Pharmacogenetic Study of Clopidogrel
Molecular Diagnosis

Pharmacogenetic Study of Clopidogrel

Updated On Jul 30, 2017


The main goal of this research was to establish an easy, swift and inexpensive multiplex PCR-based detection method for four SNPs- CYP2C19*2, CYP2C19*3, CYP2C19*4 and CYP2C19*17 in the human genome. The assay method was able to classify an individual as Poor (PM)/ Intermediate (IM)/ Extensive (EM)/ Ultra Rapid Metabolizer (UM) of Clopidogrel, an anti-platelet drug prescribed to prevent internal blood clot.

 

This project also determined the frequency of these four alleles in Bangladeshi population using 150 volunteers.

 

This project work includes:

• collection of buccal swabs from donors and extraction of DNA via Chelex method

in silico designing of allele specific primers targeting four SNPs in the human CYP2C19 gene

• Multiplex PCR and Agarose Gel Electrophoresis



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